端粒、端粒酶及其亚单位在鼻咽癌中相互作用的研究

The role of telomere, telomerase and its hTR in nasopharyngeal carcinoma

目的 探讨端粒、端粒酶及其亚单位在鼻咽癌发病中的作用。方法 采用Southern杂交等分子生物学方法对鼻咽癌端粒、端粒酶及其亚单位进行检测。结果 鼻咽癌平均端粒长度 (MTL)为 4 .5± 2 .3kb ,端粒酶阳性表达为 88.0 %～ 10 0 %。对照组MTL分别为 14 .6± 2 .8Kb和 15 .8± 3.8Kb ,癌旁组织端粒酶阳性表达为 6 4.7% ,对照组为 0～ 8.7%。此外 ,端粒酶活性增高与晚期鼻咽癌及其颈淋巴结转移也有密切关系。结论 端粒及端粒酶与鼻咽癌的发生。

Objective To explore the role of telomere, telomerase and its hTR in NPC.Methods The telomere kinetic, The expressions of telomerase and its hTR were studied with Southern Blot, TRAP PCR ELISA and RT nested\|PCR, respectively.Results There were shorter MTL (4.5±2.3kb) and higher telomerase activity (88.0%) in nasopharyngeal tissues as compared with normal control. High expression of telomerase was also noticed in the adjacent tissues( 64.7%). Telomerase activities were also related to the late stage carcinoma and NPC with neck lymph node involvement. Conclusion It may be closely related to the occurrence, development and metastasis of NPC for telomere kinetic and telomerase activity.