宫颈癌细胞瘤苗的主动免疫治疗研究

Experimental study of active specific immunotherapy for cervic cancer with tumor vaccine

目的 研究添加新型佐剂的宫颈癌细胞肿瘤瘤苗的主动免疫治疗效果。方法 用Hela细胞重组白细胞介素 2、重组粒细胞单核细胞集落刺激因子、特制中药提取物及福氏不完全佐剂制成疫苗 ,测定瘤苗免疫组、荷瘤组、正常组小鼠脾细胞宫颈癌细胞的杀伤活性 ;检测血清中IL 10、IFN γ水平 ;并计算存活期。结果 效靶比为 2 0 0 :1时 ,免疫小鼠脾细胞体外杀伤宫颈癌细胞的杀伤率为 39% ,显著高于荷瘤组的14 % ,正常组的 8%。瘤苗免疫组血清IFN γ较其余组明显升高 (P <0 .0 1) ;血清IL 10较其余组明显降低(P <0 .0 1)。同时 ,瘤苗免疫组生存期亦有明显延长。结论 新型肿瘤细胞瘤苗可激发特异性细胞介导的免疫反应 。

Objectives To evaluate the active immunotherapeutic efficacy of tumor vaccine with new adjuvants. Methods HeLa cells, rIL\|2,rGM\|CSF,traditional Chinese medicine and Feud's adjuvant were mixed together to form tumor vaccine. An animal model of tumor vaccination was established. The 51 C r release assay was used to examine the cytotoxicities of three kinds of the splenocytes to cervic cancer cells. Serum IL\|10, IFN\|γ were tested. The other mice in every group were fed continously to calculate survival time. Results Specific cytocidal rate of splenocytes from the immunized mice to cervic cancer cell was 39% when E:T was 200:1,which was significantly higher than that from the other two groups. Serum IFN\|γ in immunized group was increased obviously compared with other groups( P <0.01). The survival time of the mice in immunized group were prolonged. Conclusions The novel tumor vaccine can improve the host's antitumor immune response.