和解汤对慢性乙型肝炎T细胞受体Vβ7基因表达的影响

Effect of Hejie Decoction on T-Cell Receptor Vβ_(7) Gene Expression in Patients of Chronic Hepatitis B

目的:探讨和解汤对慢性乙型肝炎（简称慢乙肝）临床疗效与T细胞受体Vβ7（TCRVβ7）基因表达特征的相关性。方法:将慢乙肝患者45例随机分为两组,治疗组（30例）采用和解汤治疗,对照组（15例）采用常规西药治疗,观察治疗后TCRVβ7,基因表达变化。结果:经过6个月治疗,两组ALT水平均显著下降（P<0.01）,治疗组5例测出TCRVβ7,基因表达,并均出现HBV-DNA、HBeAg阴转;对照组无一例测出TCRVβ7基因表达,无一例出现HBV-DNA、HBeAg阴转。HBV-DNA、HBeAg未阴转的患者,虽肝功能恢复正常,但无一例测出TCRVβ7基因表达。两组总有效率比较差异无显著性,但两组显效率比较差异有显著性（P<0.01）。结论:和解汤对TCRVβ7基因表达有调节作用,可能是抑制病毒复制、清除病毒的重要途径。

Objective: To explore the therapeutic effect of Hejie Decoction (HJD) in treating chronic hepatitis B and its relationship with T-cell receptor V***7(TCRV***7) gene expression. Methods: Forty-five patients of chronic hepatitis B were randomly divided into two groups. The 30 patients in the treated group were treated by HJD, and the 15 patients in the control group were treated by conventional western medicine. The therapeutic effect and changes of TCRV***7 gene expression after treatment were observed. Results: After 6 months treatment, the ALT level in the two groups were obviously decreased (P<0.01) . No significant difference was shown in comparison of the total effective rate between the two groups but it did show in comparison of markedly effective rate between them. TCRV***7 expression was detected in 5 patients of the treated group, and HBV-DNA and HBeAg in the 5 patients were all negatively converted. While in the control group, no one had TCRV***7 expression detected, either no one with negative conversion of HBV-DNA and HBeAg. TCRV***7 could not be detected in all the patients whose HBV-DNA and HBeAg hasn't negatively converted, though their liver function could be normalized. Conclusion: HJD might have the effect of regulation on TCRV***7 expression, it possibly is the important way for HBV replication inhibition and virucidal action of HJD.