p16基因和增殖细胞核抗原在胃癌组织中的表达与关系

p16 gene,PCNA expression and significance in primary gastric carcinoma

目的 观察胃癌组织中 p16基因的表达和增殖细胞核抗原 (PCNA)染色强度指数间的关系及临床意义。方法 采用LSAB免疫组织化学法。结果 在 34例原发性胃癌组织中 ,p16基因阳性表达率4 7 0 6 % (16 / 34) ,PCNA染色指数平均为 5 9 2 8± 3 4 8,p16基因阳性率和PCNA染色指数在组织学分级Ⅰ级中明显低于组织学分级Ⅲ级 (均P <0 0 5 ) ;在无淋巴结转移者中明显低于有淋巴结转移者 (分别为P <0 0 5 ,P <0 0 1)。结论 胃癌组织中 p16基因的表达和PCNA染色指数与胃癌的发生、发展密切相关 ,可以客观反映胃癌的组织分化状态。

Objective To observe the relationship between the expression of p16 gene and the indices of proliferating cell nuclear antigen in primary gastric carcinoma.Methods Immnuohistochemistry of labelled streptavidin biotin method was used in this study.Results The positive rate of p16 gene and mean indices of PCNA in the group with histological grade Ⅰ but without lymphaden metastasis were significantly lower than those in the group with histological grade Ⅲ and lymphaden metastasis(P<0.01,P<0.05).Conclusion The expression of p16 gene and indices of PCNA might be related to the genesis and development of gastric carcinoma.High levels of p16 expression and indices of PCNA in gastric carcinoma may be associated with poor differentiation,rapid progression,and high risk of lymphaden metastasis.