HPLC法对唐古特瑞香中瑞香素和芫花素含量的测定

Determination of Daphnetin and Genkwanin in Daphne tangutica by HPLC Method

建立同时测定唐古特瑞香（Dap hne tangutic a）中瑞香素和芫花素的含量的HPLC方法。采用Agi-lent ZORBAX（4.6 mm×250 mm，5μm）色谱柱；检测波长为360 nm；柱温为30℃。结果表明，瑞香素在0.115~0.920μg内线性关系良好（R^2=0.9999），其平均回收率为99.19%，RSD=1.2%（n=9）。芫花素在0.011~0.088μg内线性关系良好（R^2=0.9999），其平均回收率为100.25%，RSD=1.5%（n=9）。该方法样品处理简单、准确度高、分离效果好，适用于该药的成分测定。

The high performance liquid chromatography （HPLC﹚ was used to simultaneously determine daphnetin and genkwanin in Daphne tangutica. The samples were separated on Agilent ZORBAX （4.6 mm×250 mm,5μm﹚ column. Detection wavelength was 360 nm and column temperature was 30℃. The results showed that the linear range of daphnetin was good in 0.115~0.920μg （R^2=0.999 9﹚ with the average recovery of 99.19% and RSD of 1.2%（n=9）. The linear range of genkwanin was good in 0.011~0.088μg （R^2=0.999 9﹚ with the average recovery of 100.25% and RSD of 1.5%（n=9﹚. This method was simple, accurate,and suitable for determining components and quantities of daphnetin and genkwanin in Daphne tangutica.