奶牛TNMD基因的克隆与原核表达

Cloning and Prokaryotic Expression of Bovine TNMD Gene

从奶牛组织中克隆Tenomodulin(TNMD)基因的cDNA序列,采用双酶切后连接表达载体pGEX-4T-1,转入大肠杆菌BL21中进行诱导表达。结果表明,TNMD基因的序列全长为957 bp,通过双酶切构建的表达载体pGEX-TNMD在BL21大肠杆菌中成功表达了分子量为59.68 kDa的融合蛋白。

The Tenomodulin （TNMD﹚ cDNA of bovine was amplified by RT-CR. Then the gene fragment was digested with enzyme and cloned into expression vector pGEX-4T-1. The recombinant vector was transformed into E. coli BL21. The results showed that the full-length of the TNMD gene sequence was 957 bp. The reconstruction plasmid pGEX-TNMD was constructed successfully and a 59.68 kDa fusion protein was expressed in E. coli BL21 induced by IPTG.