结核分枝杆菌Rv3407蛋白抗原表位的预测

Prediction of epitopes of Mycobacterium tuberculosis Rv3407 protein

目的预测结核分枝杆菌Rv3407的抗原表位。方法利用DNAStar软件包中Protean软件对Rv3407氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位。结果 Rv3407蛋白具有丰富的二级结构和多处抗原指数较高的区段,潜在的B细胞抗原表位较少,可能位于11-26、28-43、50-61、66-74、76-99位氨基酸残基或其附近,这些区域基本上含有β转角结构,亲水性、表面可能性和柔韧性指数都较高。该蛋白潜在的T细胞抗原表位较多,可能位于2-12、22-26、34-37、40-44、56-60、75-79、86-93位氨基酸残基或其附近。结论结核分枝杆菌Rv3407是一个T细胞抗原表位占优势的蛋白抗原,B细胞抗原表位略少,预测结果为该蛋白抗原表位的进一步研究与应用奠定了基础。

Objective To predict the epitopes of Mycobacteriurn tuberculosis Rv3407 protein. Methods The amino acid sequence of Rv3407 protein was analyzed using Protean in DNAStar and B cell and T cell epitopes were predicted using multiple parameters, including the secondary structure, hydrophilicity, antigenicity, surface probability, and flexi-bility. Results The Rv3407 protein has an extensive secondary structure and multiple regions with high antigenicity. There were a few potential B cell epitopes at or near amino acids 11--26, 28--43, 50--61, 66--74, and 76--99. Theses epitopes had greater antigenicity, a beta turn, and a high level of hydrophilicity, surface probability, and flexibility. There were potential T cell epitopes at or near amino acids 2--12, 22--26, 34--37, 40--44, 56--60, 75--79, and 86-- 93. Conclusion M. tuberculosis Rv3407 protein is an antigen with a dominant T-cell epitope, B cell epitopes also exist but are less prevalent than T cell epitopes. This prediction of epitopes has laid the foundation for the further study and use of this protein.