电离辐射多房棘球蚴病理组织学观察

Histopathology of Echinococcus multilocularis after exposure to ionizing radiation

目的重离子对肿瘤放疗比常规放疗更具优势,因为重离子放疗具有极佳的生物效应和剂量一致性。多房棘球蚴病具有肿瘤特性,本研究主要目的是用重离子放疗作为多房棘球蚴病的一种非手术治疗方法。方法通过LD50来评价原头蚴的死亡情况,应用光镜和投射电镜研究多房棘球蚴经X射线和碳离子电离辐射照射后形态结构变化。结果电离辐射使多房棘球蚴细胞质减少,生发层细胞深入到角质层内的绒毛消失。细胞器混乱并聚集,线粒体、高尔基复合体等细胞器大量消失,生发层细胞内出现大液泡。与X线相比,碳离子辐射对多房棘球蚴的抑制作用更为明显。结论多房棘球蚴经电离辐射后细胞结构和超微结构发生巨大变化,提示电离辐射可抑制多房棘球蚴生长。碳离子电离辐射对多房棘球蚴的抑制作用比X线辐射照射更为明显。

Objective Heavy ion therapy has an advantage over conventional radiotherapy due to its superb biological effectiveness and dose conformity in cancer therapy. Heavy ion therapy could be a potential way to treat Echinococcus ultilocularis because E. multilocularis resembles a tumor. Methods Protoscolex death was assessed using the LD50. Ultrastructural morphological changes within the parasite were studied using light and electron microscopy. Results Ionizing radiation induced a sparse cytoplasm, disorganized and clumped organelles, extensive absence of mitochondria and Golgi complexes, large vacuoles, and absence of villi. Cyst apoptosis was pronounced after radiation. Carbon ion radiation was more effective at inhibiting hydatid cysts than X-rays. Conclusion The current findings provide a framework to evaluate the attenuating effect of heavy ion radiation on cystic echinococcosis in vitro. Carbon ion radiation is more effective at in hibiting E. multilocularis than X-rays.