摘要
目的探讨鱼藤素对人宫颈癌HeLa细胞生存活力和凋亡的影响。方法将不同浓度(0、10、100、500、1 000 nmol/L)的鱼藤素作用于HeLa细胞24、48和72 h,磺基罗丹明B(sulforhodamine B,SRB)法检测细胞的生存活力;Hoechst 33258染色法观察细胞形态变化;Annexin V-FITC双标法检测细胞凋亡;流式细胞仪检测细胞周期变化。结果鱼藤素对HeLa细胞的生存活力具有明显抑制作用,且呈现出时间和剂量依赖性;流式细胞仪检测发现鱼藤素对HeLa细胞有诱导凋亡并使细胞周期阻滞在G0/G1期的作用。结论鱼藤素在体外能有效抑制HeLa细胞的生存活力并诱导其凋亡。
【Objective】To investigate the effect of deguelin on viability and apoptosis of human cervical cancer HeLa cell. 【Methods】 Using different concentrations(0, 10, 100, 500, 1 000 nmol/L) of deguelin on HeLa cells at 24 h, 48 h, 72 h. SRB assay was used to detect the cell viability of each group at different times; Hoechst 33258 staining assay was used to observe the morphological changes induced by deguelin; Annexin V-FITC double labeled flow cytometry was used to detect the apoptosis; the cell cycle was analyzed via flow cytometry. 【Results】 The viability of HeLa cells was inhibited by deguelin in a time and dose dependent manner. The flow cytometry results showed that deguelin could induce HeLa cells apoptosis and the cell cycle was arrested in G0/G1 phase. 【Conclusion】 Deguelin can efficiently inhibit the viability and induce apoptosis of Hela cells in vitro.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第17期10-14,共5页
China Journal of Modern Medicine