食源性微生物的分子生物学检测方法的研究进展

Advance in Detection Method of Molecular Biology for Foodborne Microorganisms

食源性微生物污染是食品安全中最突出的问题,深切地影响着大众的消费心理与身心健康。在诸多的检测技术中,传统PCR技术、传统PCR衍生的PCR技术、荧光定量PCR技术、LAMP技术、DNA杂交技术、DNA序列分析技术以及基因芯片技术等分子生物学检测技术具有敏感、特异、简便、快速等特点,可实现对食品中微生物的快速检测,为食品安全提供强有力的监控手段。就食源性微生物的分子生物学检测方法进行简要综述。

The pollution caused by foodborne microorganisms is the most dominant issue for food safety, which has deeply affected consumer psychology and physical and mental health of the public. During many detection methods, detection technologies of molecular biology including traditional PCR, derivated PCR, real-time PCR, LAMP, DNA hybridization, DNA Sequence Analysis and gene chip have characteristics of sensible, specific, convenient and simple and rapid, which can detect pathogenic microorganisms in food to provide forceful technical means. Main detection methods of molecular biology used for foodborne microorganisms would be concisely summarized.