红景天8种成分体外抗氧化作用比较研究

Comparison on the Antioxidant Activity of Eight Components from Rhodiola in Vitro

目的:比较性研究红景天中8种成分,主要包括芦丁苷、红景天苷、异槲皮苷、酪醇、莨菪亭、熊果苷、山奈酚、肉桂醇红景天,并进行体外抗氧化实验。方法:测定8种成分对二苯代苦味酰基自由基(DPPH)、超氧阴离子自由基、一氧化氮自由基、羟自由基的清除能力,以及总抗氧化能力和抑制脂质过氧化反应的能力。结果:除清除羟自由基实验外,异槲皮苷和芦丁苷作用明显强于其他化合物,其中在清除DPPH、超氧阴离子能力和抑制脂质过氧化实验中的IC50分别为6.43 mg·L-1和11.56mg·L-1,24.19 mg·L-1和37.25 mg·L-1,11.76 mg·L-1和57.24 mg·L-1;而在清除羟自由基实验中,酪醇和红景天苷的作用则强于其他化合物,IC50分别为11.76 mg·L-1和19.5 8 mg·L-1。结论:红景天中苷类及醇类成分对多种自由基具有清除作用,有良好的综合抗氧化性和开发应用前景。

Objective： To comparative study of rhodiola eight components,mainly including rutin glycosides,rhodiola glucoside,isoquercitrin,butyl alcohol,belladonna pavilion,arbutin,kaempferol,cinnamyl alcohol rhodiola,and the in vitro antioxidant effect. Methods： determination of eight ingredients on DPPH free radical,superoxide free radicals,nitric oxide free radical,hydroxy free radical removal ability,and total antioxidant capacity and inhibit lipid peroxide reaction ability. Results： in addition to clear hydroxy free radical experiment outside,isoquercitrin and rutin glycosides effect is stronger than the other compounds,which in a clear DPPH,super oxygen anion ability and inhibit lipid peroxide in the IC50 were 6. 43 and 11. 56,24. 19 and 37. 25,11. 76 and 57. 24 mg·L- 1; And in clear hydroxy free radical experiments,dairy and rhodiola I effect is better than other compounds,IC50 were 11. 76 and 19. 58 mg·L- 1. Conclusion：The rhodiola glycosides and alcohol composition in a variety of free radical scavenging effect,has good comprehensive oxidative stability and development and application prospect.