栀子蓝色素的制备及其抗氧化活性研究

Study on the preparation of gardenia blue and its antioxidant activity

用米曲霉发酵产物水解栀子苷，水解产物与味精反应制备栀子蓝色素，采用DM-2大孔树脂对产品进行精制，通过DPPH·捕获分光光度法测定栀子蓝色素精制前后的抗氧化活性，栀子蓝制备的最佳条件为：10．64g栀予苷和129．36g水混合后，加入米曲霉发酵液9．8mL，在50℃，160—180r／min下水解6h后，与3．15g味精反应40h，80℃保温30min,所获栀子蓝色素粗品的色价（E1cm^1%）为90．56，经DM-2大孔树脂纯化后产品色价提高至180．01％，收率为90．96％,抗氧化实验表明栀子蓝色素粗品和精制品均能有效清除DPPH·，且精制后的栀子蓝色素产品清除DPPH·的能力增强。

Gardenoside is hydrolyzed with fermentation broth of Aspergillus oryzae and reacted with monosodium glutamate, which led to the synthesis of gardenia blue. Gardenia blue is purified by DM -2 macroporous resin column chromatography. Scavenging DPPH · free radical assay for gardenia blue is carried out. The results have indicated the preferred preparation condition of gardenia blue is that a mixture of 10. 64 g geniposide,129. 36 g H20 and 9. 8 mL fermentation broth of Aspergillus oryzae is hydrolyzed at 50 ℃ under shaking at 160-180 r/min rotation of 6 h and then reacted with 3. 15 g monosodium glutamate at the same temperature for 40 h and continued to react at 80 ℃ for 30 min. E1cm^1% （color value） of the crude reaction product reaches 90. 56. The crude reaction product is chromatographied on the macroporous resin column to get gardenia blue with a production rate of 90.96% and an E1cm^1%of 180.01. Purified gardenia blue has showed a good DPPH free radical scavenging effect in DPPH free radical assay, which is stronger than that of the crude reaction product.