摘要
目的探讨赤芍总甙对PC12细胞钙超载损伤的保护作用及其机制。方法采用组织培养法 ,以大鼠肾上腺嗜铬细胞瘤克隆株PC12细胞为材料 ,制备KCl及NMDA诱导的钙超载损伤模型。结果形态学检查发现赤芍总甙对两种不同类型的钙超载损伤模型中PC12细胞均具有明显保护作用 ,MTT法活细胞测定提示赤芍总甙可显著提高损伤模型中PC12细胞存活数 ,减少胞内乳酸脱氢酶渗漏 ,并显著减少胞内钙离子浓度。结论赤芍总甙对钙超载所致PC12细胞损伤有显著的保护作用。
Aim The protective effect and mechanism of TPG on PC12 cells in calcium overloading injury models were studied. Methods Two injury models induced by KCl and NMDA were used to assay the action of TPG in cultured PC12 cells. Results The morphological examination revealed that TPG possessed obvious protective effects on PC12 cells in injury models. MTT and LDH measurement indicated that TPG increased the number of live cells and reduced the extent of cell injury significantly. TPG also lessened the concentration of calcium ion in cytoplasm. Conclusion TPG protected rat PC12 cells against two calcium overloading injuries effectively in vitro. Its actions may deal with anti oxidation, inhibition of NO production and blocking of both types of calcium channel.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2000年第2期120-123,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
