光照变化对小鼠褪黑素影响的实验研究

Experimental study on illumination changes affecting melatonin of mice

目的从光照变化对小鼠褪黑素影响,探索昼夜阴阳消长与生物节律的关联性。方法雄性昆明小鼠144只,随机分为为正常组、长光照组、长黑暗组3组,每组各48只。适应性饲养7天,光照控制干预6周后开始试验取材,分别于0：00,4：00,8：00,12：00,16：00,20：00共6个时间点分组取血,检测血中褪黑素。对各组各时间点小鼠褪黑素数据进行方差分析确定昼夜节律的存在;并分析不同光照条件下小鼠褪黑素节律的不同。用LSD方法对各时间点数据进行两两比较分析,分析昼夜节律对小鼠褪黑素的影响。结果（1）同组小鼠不同时间点血清褪黑素含量有统计学差异（P〈0.05）,但其节律性随着光照不同呈现不同的特点。（2）正常组小鼠血清褪黑素含量4：00最高。20：00～4：00是血清褪黑素含量升高的转折时段,4：00～8：00是血清褪黑素含量降低的转折时段。（3）长光照组小鼠血清褪黑素含量0：00、4：00、8：00这3个时间点含量较高,三者比较差异无统计学意义。12：00、16：00、20：00这3个时间点含量较低,三者比较差异无统计学意义。8：00～12：00是血清褪黑素含量明显降低的转折时段,20：00～0：00（24：00）是血清褪黑素含量明显升高的转折时段。（4）长黑暗组小鼠血清褪黑素含量0：00、4：00两个时间点较高,两者比较差异无统计学意义。8：00、12：00、16：00、20：00这4个时间点含量较低,四者比较差异无统计学意义。在4：00～8：00时间段是血清褪黑素含量明显下降的转折时段,在20：00～0：00点时间段是血清褪黑素含量明显上升的转折时段。（4）长光照组褪黑素含量均值与正常组进行组间比较,小鼠血清褪黑素整体水平上升,P〈0.05,差异有统计学意义;长黑暗组褪黑素含量与正常组进行组间比较,小鼠血清褪黑素整体水平无明显变化,P＞0.05,差异无统计学意义。结论（1）昼夜不同光照改变,可以影响小鼠褪黑素的昼夜节律,导致其昼夜相位或整体含量的改变,这可能是现代光照条件下节律紊乱性疾病发生的重要因素之一。（2）中医关于昼夜阴阳消长对人体生理病理影响的天人相应理论具有客观物质基础和科学内涵。

Objective To explore the relationship between the wax and wane of Yin and Yang during night and day and biological rhythm through illumination changes affecting melatonin of mice. Meth-ods 144 male kunming mice were randomly divided into normal group,long light group and long dark group ,48 mice each group. Adaptive breeding for 7 days,after intervention with lighting control six weeks,taking sample of blood in six time points （0：00,4：00,8：00,16：00,12：00,20：00）respectively and detecting the content of melatonin . Using ANOVA variance analysis to determine the existence of circa-dian rhythm,and analysing the different illumination conditions,different mouse melatonin rhythm. Use LSD method to analyse each time point of data for the two two comparative analysis,analyse of the impact of cir-cadian rhythm of melatonin in mice. Results （1）There were significant differences in the same group at different time points in serum melatonin content （P〈0.05 ）,but the rhythm with the light show different characteristics are different. （2）The normal mice serum melatonin content is highest at 4：00 ,20：00～4：00 is a turning period of elevated serum melatonin content,4：00～8：00 is the turning period of decreased serum melatonin content. （3）The 3 time point of mice serum melatonin content in long photoperiod group at 0：00,4：00,and 8：00 is high,no statistically significant difference between the three. 12：00,16：00, 20：00 this 3 time points,serum melatonin content is low,no statistically significant difference between the three. 8：00～12：00 is a turning period of serum melatonin content decreased,20：00～0：00 （24：00）is a turning period of serum melatonin levels markedly increased. （4）As for comparison of average concentra-tion of melatonin between the long photoperiod group and the normal group ,the overall level of serum me-latonin increased in mice,P 〈0.05,the difference is statistically significant;Compared with melatonin content between long dark group and normal controls group,no significant change in the overall level of ser-um melatonin in mice,P〉0.05,no significant difference. Conclusion （1）The circadian change of dif-ferent illumination influencing the circadian rhythm of melatonin in mice and leading to changes in the cir-cadian phase or the whole content,which may be one of important factors inducing rhythm disorder disease owing to modern light exposure. （2）The human-heaven corresponding theory of Chinese medicine on the effect of circadian growth and decline of yin and yang to human physiology and pathology has the objective material basis and scientific connotation.