玉米秸秆瞬间蒸汽爆破预处理的回归优化和结构分析

Regression optimization and structural analysis on instaneous steam explosion pretreatment of corn stalk

采用响应面法回归分析优化瞬间蒸汽爆破预处理玉米秸秆过程,研究了汽爆压强、维压时间以及填料量三因素对酶解糖产率的影响,基于Box-Behnken设计,分析并获得了一个二阶线性方程模型,能够较好地拟合实验值。获取的最优条件为汽爆压强3.5MPa,维压时间50s,填料量60g,此时糖产率达到54.37%,相比于未处理物料,其糖化率提高了1.88倍。采用扫描电镜、X射线衍射分析以及傅里叶红外光谱对处理前后的物料进行结构和组分分析,与未处理的物料相比,处理后的物料结晶度明显降低,颗粒度减小,可及度显著提高。

This study aimed to perform a response surface methodology （RSM）regression analysis which led to the optimization on the operation condition of instaneous steam explosion pretreatment on corn stalk.The effects of pressure,time and filling quantity on total sugar yield obtained from enzymatic hydrolysis of pretreated corn stalk were investigated.Based on Box-Behnken design,a second order polynomial equation mode was obtained which can fit well the experment values.The optimized conditions for instaneous steam explosion pretreatment were 3.5 MPa for pressure,50 s for time and 60 g for filling quantity with a sugar yield of 54.37%.Compared with the untreated materials,the saccharification rate increased 1.88 times.Compared with the untreated corn stalk,the pretreated corn stalk was found to be porous,less crystalline and favorable to enzymatic hydrolysis based on the structural and component analyses on the treated and untreated materials by scanning electron microscopy （SEM）,X-ray diffraction （XRD）and Fourier transform infrared spectroscopy （FTIR）.