黄金梨“铁头病”果肉组织切片观察

Microscopic Observation of the ‘Hard-end Disorder' on the Pulp Tissue of ‘Hwangkumbae' Pear

以黄金梨为试材,采用显微切片染色技术,对黄金梨"铁头病"发病部位果肉组织中果胶、纤维素、胼胝质、淀粉等物质进行了切片染色观察。结果表明:与正常果相比较,发病果实果肉石细胞密度大、体积大,纤维含量高,而胼胝质无明显差异;淀粉粒增大,果胶含量高,在靠近果皮部形成团块状物质。试验表明,黄金梨"铁头病"的发生与果肉中石细胞大小、果胶含量和纤维素等物质变化相关,纤维素和果胶增多可能是引起果肉硬化的原因之一。

Taking ‘Whangkeumbae＇ as materials,by using the microscopic section dyeing technology,the contents of pectin,cellulose,callose and starch in the flesh tissue of‘Whangkeumbae ＇pear fruit with or without ‘hard-end disorder＇ were observated.The results showed that disease fruit had a high stone cell density,big volume and high cellulose content comparison with normal fruit.The content of callose had no obvious difference between diseased fruit and normal fruit.In addition,the contents of starch grains and pectin in diseased fruit were increased,and near the skin forming block mass compared to normal fruit.These results indicated that‘hard-end disorder＇of‘Whangkeumbae＇was related to cell size,pectin and cellulose contents of flesh tissue.The cellulose and pectin increase of flesh tissue may be one of the causes of pulp hardening.