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5-氮杂胞苷对石斛生物活性成分的影响 被引量：8

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摘要 本研究利用5-氮杂胞苷(5-azaC)处理石斛组培苗,分析石斛苗生长变化、生物活性物质含量及其相关基因表达变化。研究发现,石斛苗生物量和生物活性物质,如石斛多糖、石斛碱和胡萝卜素,含量明显升高,相关基因表达量也显著上升,证实DNA去甲基化修饰对石斛次生代谢产物生物合成具有重要调控作用。
作者 倪竹君 殷丽丽 应奇才 茹雅璐 王迪仙 金琳 谢军 胡忠建 王慧中 徐祥彬
机构地区 杭州师范大学生命与环境科学学院
出处 《浙江农业科学》 2014年第7期1018-1020,共3页 Journal of Zhejiang Agricultural Sciences
基金 杭州市科技发展计划项目(20120232B11)
关键词 5-氮杂胞苷 DNA去甲基化修饰 石斛 生物活性成分
分类号 S432.2 [农业科学—植物病理学]
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参考文献8

  • 1华扬,陈学峰,熊建华,张义平,朱英国.水稻冷胁迫诱导的甲基化差异片段CIDM7的分离和分析[J].遗传,2005,27(4):595-600. 被引量：42
  • 2Kovarik A, Koukalova B. Hypermethylation of tobacco heterochromatic loci in response to osmotic stress [ J ]. Theor Appl Genet, 1997, 95:301-306.
  • 3Law J A, Jacobson S E. Establishing, maintaining and modifying DNA methylation patterns in plants and animals [ J]. Nature, 2011, 11 (3): 204-220.
  • 4Soppe W J, Jacobsen S E, Alonso-blanco C, et al. The late flowering phenotype offwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene [ J]. Mol Cell, 2000, 6:791 -802.
  • 5Zhu J K. Active DNA demethylation mediated by DNA glycosylases [J]. Annu Rev Genet, 2009, 43:143-166.
  • 6Zhu J K, Kapoor A, Sridhar V V, et al. The DNA glycosylase/ lyase ROS1 functions in pruning DNA methylation patterns in Arabidopsis [J]. Curr Boil, 2007, 17 (1): 54-59.
  • 7Mooibroek H, Kuipers A G, Sietsma J H, et al. Introduction of hygromycin B resistance into Schizophyllum commune: preferential methylation of donor DNA [ J ]. Mol Gen Genet, 1990, 222 (1): 41-48.
  • 8Cheng J C, Matsen C B, Gonzales F A, et al. Inhibition of DNA methylation and reactivation of silenced genes by zebularine[J]. J Natl Cancer lnst, 2003, 95 (5): 399 - 409.

二级参考文献20

  • 1Finnegan E J, Peacock W J, Dennis E S. DNA methylation,a key regulation of plant development and other processes. Curr Opion Genet Develop ,2000,10:217～223.
  • 2Richards E J. DNA methylation and plant development. Trends Genet, 1997,13(8) :319～322.
  • 3Kass S U, Pruss D, Wolffe A P. How does DNA methylation repress transcription? Trends Genet, 1997,13:444～449.
  • 4Finnegan E J, Genger R K, Kovac K, Peacock W J, Dennis E S.DNA methylation and the promotion of flowering by vernalization. Proc Natl Acad Sci USA, 1998,95:5824 ～ 5829.
  • 5Wassenegger M, Pelissier T. A model for RNA-mediated gene silencing in higher plants. Plant Mol Biol, 1998,37:349～362.
  • 6Jaglo-Ottosen K R, Gilmour S J, Zarka D G, Schabenberger O,Thomashow M F. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science, 1998,280:104～106.
  • 7Tahtiharju S, Sangwan V, Monroy A F, Dhindsa R S, Borg M.The induction of kin gene in cold-acclimating Arabidopsis thaliana.Evidence of a role for calcium. Planta, 1997,203:442～447.
  • 8Knight H, Trewavas A J, Knight M R. Cold-calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after accliamtion. Plant Cell, 1996,8:489～503.
  • 9Xin Z, Browse J. Eskimo I mutants of Arabidopsis are constitutively freezing-tolerant. Proc Natl Acad Sci USA, 1998, 95:7799～7804.
  • 10McKown R, Kuroki G, Warren G. Cold responses of Arabidopsis mutants impaired in freezing tolerance. J Exp Bot, 1996,47:1919～1925.

共引文献41

同被引文献170

引证文献8

二级引证文献53

浙江农业科学
2014年 第7期

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