一测多评法同时测定板蓝根中4种核苷及(R,S)-告依春

Assay of epigoitrin and four kinds of nucleosides in Isatidis Radix by QAMS

目的建立同时测定板蓝根中4种核苷(腺苷、胞苷、尿苷、鸟苷)及(R,S)-告依春的HPLC-DAD定量检测方法。方法板蓝根水提取,采用Agilent 1260、Agilent 1100、Waters 2695-2996高效液相色谱仪,流动相为水-甲醇,梯度洗脱。以腺苷、胞苷和尿苷为参照成分计算色谱峰之间的相对校正因子,再与外标法比较。结果 33批板蓝根药材中5种成分相对校正因子在3种仪器检测的耐用性良好。计算值与实测值(外标法)相对误差<5%。结论本研究建立了准确、快速且耐用性良好的板蓝根中5种活性成分同时测定的"一测多评"方法,但本方法得到的相对校正因子受检测波长变化影响较大,5种活性成分同时测定的波长宜为254 nm。

AIM To establish an HPLC-DAD method for determining the contents of adenosine,cytidine,uridine,guanosine and（ R,S）-epigoitrin for the quality control of Isatidis Radix. METHODS The analysis of aqueous extract of Isatidis Radix was performed on Agilent 1260、Agilent 1100 and Waters 2695- 2996 with mobile phase consisted of water and methanol in gradient elution manner. Their relative correction factors（ RCFs） between the peaks of nucleosides and epigoitrin were calculated with adenosine,cytidine and uridine as references,as compared with the external standard. RESULTS The durability of the method was evaluated on five tested constituents from thirty-three batches of Isatidis Radix on three HPLC apparatuses,and their relative error was no more than 5%. CONCLUSION An accurate,rapid and highly durable QAMS method is established for simultaneous determination and location of the four active nucleosides and epigoitrin. Because of the RCFs of established method susceptible to wavelength detection,254 nm is recommended as one being helpful to the assay of five constituents of Isatidis Radix.