陈皮饮片的质量控制

Quality control for the herbal pieces of Citri reticulatae Pericarpium

目的探索建立陈皮饮片的质量控制方法。方法采用Agilent TC-C18（2）（4.6 mm×250 mm,5μm）,以乙腈-水为流动相,梯度洗脱,体积流量1.0 mL/min,波长范围220～375 nm,柱温30℃。结果陈皮饮片中的芸香柚皮苷、橙皮苷、甜橙黄酮、川陈皮素、橘皮素等的线性范围和相关系数分别为29.98～479.68μg/mL（r=0.999 8）,198.88～3 182.08μg/mL（r=0.999 8）,0.158～2.528μg/mL（r=0.999 8）,1.008～16.128μg/mL（r=0.999 9）,0.398～6.368μg/mL（r=0.999 8）;加样回收率分别为99.91%（RSD=1.3%）,99.59%（RSD=1.2%）,100.22%（RSD=1.3%）,99.85%（RSD=1.3%）和99.71%（RSD=1.5%）;方法精密度、重复性良好,RSD均小于2.0%。结论本法操作简便,结果可靠,重复性好,检测快速,定量准确,可作为陈皮饮片质量控制的方法。

AIM To establish a method for the quality control for decoction pieces of Citri reticulatae Pericapium. METHODS The RP-HPLC method was used for methanolic extract of Citri reticulatae Pericapium,which chromatographic conditions were C18column and photodiode-array-detector. The mobile phase was acetonitrile-water in gradient elution manner,the flow rate was 1. 0 mL /min,wavelength detection was set between 220 nm and 375 nm,the column temperature was maintained at 30 ℃. RESULTS The linear ranges of narirutin,hesperidin,sinensetin,nobiletin,and tangeretin fell within 29. 98- 479. 68 μg /mL（ r = 0. 999 8）,198. 88- 3 182. 08 μg / mL（ r =0. 9998）,0. 158- 2. 528 μg /mL（ r = 0. 999 8）,1. 008- 16. 128 μg /mL（ r = 0. 999 9） and 0. 398- 6. 368 μg /mL（ r =0.999 8）,respectively. The average recoveries were 99.91%,99.59%,100.22%,99.85% and 99.71%with corresponding RSDs of 1. 3%,1. 2%,1. 3%,1. 3% and 1. 5%,respectively. The RSDs of precision and accuracy of the assay were not more than 2. 0%（ n = 6）. CONCLUSION The method is rapid,accurate,reliable and can be used for quality control of the decoction pieces of Citri reticulatae Pericapium.