抗-E所致HDN的实验室诊断与输血选择——附1例报告

Laboratory diagnostic and blood transfusion choice of HDN induced by anti-E

目的探讨抗-E所致新生儿溶血病的实验室诊断与输血选择。方法分别用盐水、菠萝酶、抗球蛋白法鉴定患儿血清、红细胞乙醚放散液以及母亲血清中同种抗体特异性,为患儿选择相配合的血液。结果患儿血清、红细胞乙醚放散液以及母亲血清中检出IgG抗-E。结论选择同种抗体相应抗原阴性的红细胞与患儿血清及红细胞乙醚放散液进行交叉配血主侧均相合,输注后无输血不良反应。

Objective To study the laboratory diagnostic and the choice of blood transfusion of HDN induced by anti-E. Methods In order to select compatible blood for the child with HDN ,child＇s and her mother＇s serum and elution fluid from red blood ceils based on ether eluted were detected, specitively with saline test, pineapple enzymatic test and antiglobulin test. Results IgG anti-E is detected in the child and her mother＇s serum. Conclusion Alloantibody corresponding antigen neg- ative red blood cells were cross matching and consistency both with the child＇s serum and with red blood cells elution fluid at the main side cross. No adverse reaction happens after transfusion.