1株溴苯腈降解菌的分离鉴定及降解特性研究

Isolation and identification of a bromoxynil degradation strain and its degradation characteristics

从农药厂污水处理池的活性污泥中分离得到1株能够以溴苯腈为唯一碳源的菌株,命名为XBJ。对菌株XBJ进行生理生化鉴定及16S rRNA基因序列分析,研究其在不同的初始pH、温度、NaCl浓度、通气量情况下的生长情况,以及在不同的初始pH、温度、初始溴苯腈浓度、接种量情况下对溴苯腈的降解情况。结果表明:经生理生化分析以及16S rRNA基因序列比对分析,将菌株XBJ初步鉴定为Ochrobactrum sp.。菌株XBJ在30℃、pH 7.0的LB培养基中生长最佳。菌株XBJ降解溴苯腈的最适条件为:30℃、pH 7.0。当溴苯腈质量浓度为100 mg·L-1,接种量为5%时,72 h降解率达到91.87%以上。提高接种量有利于加快溴苯腈的降解,当接种量提高到10%时,72 h溴苯腈残留质量浓度为1.53 mg·L-1。

In this paper,a highly efficient bromoxynil degrading strain was isolated from the pesticide wastewater treatment pool by enrichment and separation technology,and the strain was named as XBJ.Based on its physiological and biochemical properties and16 S rRNA gene sequence analysis of the strain,the growth of the strain under different environmental conditions was studied,as well as the degradation of bromoxynil under different environmental conditions.The results showed that a bromoxynil-degrading strain XBJ was isolated from pesticide wastewater treatment pool.This strain was primarily identified as Ochrobactrum sp.based on its physiological and biochemical characteristics and 16 S rRNA gene sequence.The optimal pH and temperature for the degradation of bromoxynil by strain XBJ were 30 ℃ and pH 7.0.Strain XBJ degradated bromoxynil in 72 h by 91.87% when the initial concentration of bromoxynil was 100 mg·L-1,and with 5% inoculating quantity.Improving inoculation rate speeded up the degradation of bromoxynil.With 10% inoculating quantity,the residual concentration of bromoxynil was only 1.53 mg·L-1in 72 h.