摘要
以pCAMBIA1391Z为载体,对农杆菌EHA105介导的柠檬[Citrus limon(L.)Burm.f.]遗传转化体系进行了优化。结果表明,培养基中植物激素的浓度、农杆菌浸染浓度以及浸染后共培养的时间对柠檬的再生和遗传转化效率均有明显影响。1.0 mg/L 6-BA和0.1 mg/L的NAA有利于柠檬上胚轴不定芽的再生,并且再生芽的质量最佳。农杆菌的浸染浓度以OD600 nm=0.6为最佳,浸染后共培养2 d,在含5.0 mg/L潮霉素的筛选培养基上筛选,有利于柠檬不定芽再生率的提高。
Using pCAMBIA1391Z as vector, the genetic transformation system of lemon [Citrus limon（L.） Burm.f.]with Agrobacterium-mediated by EHA105 was optimized. The results showed that concentration of plant hormones, inoculum density and co-cultivation time had significant effects on lemon regeneration and transformation. It was most conducive to the regeneration of adventitious buds lemon epicotyl by adding 1.0 mg / L 6-BA and 0.1 mg / L NAA in MT medium. The best inoculum density and co-cultivation time were 0.6（OD600 nm） and two days. For the adventitious bud regeneration of lemon, the most benifical screening concentration of hygromycin was 5.0 mg / L.
出处
《湖北农业科学》
北大核心
2014年第9期2100-2102,2106,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金面上项目(31071786)
湖北省农业科技创新中心资助项目(2011-620-001-03)
