贵定鸟王茶和福鼎茶中咖啡因和儿茶素含量比较

The Comparative Researches of Contents of Caffeine and Catechins between Niaowang Tea and Fuding Tea in Guiding Country

利用高效液相色谱法测定贵定县本地茶种鸟王茶与引进的福鼎茶茶叶中的咖啡因和儿茶素含量,分析比较两种茶叶中咖啡因和儿茶素含量的差异,可为鸟王茶的保护与开发提供科学依据。采用C18色谱柱,流动相为NH4AC水溶液和甲醇,梯度洗脱,检测波长220 nm,流速为0.5 mL/min,柱温室温。结果表明,贵定鸟王茶与福鼎茶中的咖啡因含量无明显差异,鸟王茶儿茶素的单体化合物的含量明显高于福鼎茶。

The content of caffeine and catechins in Niaowang tea and Fuding tea were determinated by high performance liquid chromatography method. Niaowang tea was the local tea of Guiding country and Fuding tea was imported. The content of two ingredients in the two teas were analyzed and compared to provide the scientific basis for the protection and development of Niaowang tea. The separation of catechins and caffeine was achieved on a C18analytical column. The mobile phase was consisted of NH4AC aqueous solution（A） and methanol（B）. Gradient elution was performed with the wavelength at 200 nm,the flow rate of 0.5 mL / min and the column temperature of room temperature. The results showed that the content of caffeine in the two teas had little difference. The content of catechins of Niaowang tea was higher than that of Fuding tea.