HPLC-MS/MS法检测化妆品中的萘酚

HPLC-MS/MS detection of naphthol in cosmetics

建立了多反应监测法测定化妆品中α-萘酚和β-萘酚2种禁用着色剂的高效液相色谱-二级质谱联用（HPLC-MS/MS）的分析方法.针对几类美妆用品进行了样品前处理、色谱分离条件和二级质谱条件的研究.色谱条件经优化后确定：以常用的反相键合的十八烷基为固定相,以乙腈和水的二元流动相进行梯度洗脱,以配有电喷雾离子源的二级质谱为检测器等.考察了膏霜类、油状液体类和水类不同基质化妆品中两种萘酚异构体的加标回收率,标准偏差和变异系数.在0.05-10.0mg/L的范围内,2种目标物均有良好的线性关系,检测限（S/N≥10）均为0.2ng/g.6个样品的高、中、低3个标准添加水平下,回收率为81.95%-98.30%,相对标准偏差为0.001 5%-0.078%,变异系数为0.83%-8.2%.样品中均未发现禁用着色剂萘酚的2种异构体.该方法可用于化妆品中α-萘酚和β-萘酚的检测.

the study established an analytical method of multi-reactions monitoring the HPLC-MS/MS of the two forbiden colorantsα-naphthol andβ-naphthol in cosmetics,and conducted a research into the sample pretreatment,chromatograph condition and the secondary mass spectrum condition of the three beauty makeup products.After the chromatographic condition optimization it can be confirmed that the frequently-used reverse phase bonding octadecyl is used as the stationary phase,the binary mobile phase of acetonitrile and water is gradient eluted,the secondary mass spectrometry equipped with the electrospray ion source is uesd as the detector and etc.The study investigated the labelling recovery,standard deviation and variable coefficient of the two naphthol isomer in different substances cosmetics of creams,oily liquid and water-like type.Within the scope of 0.05-10.0mg/L,the two target object have a good linear relation,the limits of detection（S/N≥10）are both 0.2ng/g.Under the high,medium and low standard addition levels of 6 samples,the recovery is 81.95%-98.30%,the relative standard deviation is 0.001 5%-0.078 2%,the variable coefficient is 0.83%-8.18%.In every sample the two isomers of the forbiden colorant naphthol are not found.This method can be applied in the detection ofα-naphthol andβ-naphthol in cosmetics.