GC-MS法鉴定团花绢螟性信息素成分的研究

GC-MS study on the components of sex pheromone in Diaphania glauculalis Guenée

团花绢螟Diaphania glauculalis Guen6e是危害团花树的重要害虫。为研究利用其性信息素防控团花绢螟，在光周期L：D=12：12、温度20～28℃、相对湿度75％-80％条件下，观察了雌蛾释放性信息素情况，采用腺体提取法、吸附法、固相微萃取法制备了同日龄在不同暗期时辰、不同日龄在同一暗期时辰的雌蛾性信息素粗提物。采用GC—MS技术，计算机检索分析和鉴定性信息素成分，结果表明：3日龄雌蛾暗期处理6h产生和释放的性信息素量最大，随后逐渐减少，而吸附法、固相微萃取法均未发现性信息素组分。

Diaphania glauculalis Guen6e（Lepidoptera：Pyraustinae） is an important pest attacking Anthocephalus chinensis. A series of studies on the sex pheromone of the pest was made to find a pollution-free and efficient control method. Calling behavior of the female moth was observed at 12L： 12D photoperiod, 25-28℃ and 75%-80% RH. The crude extracts of sex pheromone were prepared with three methods. The sex pheromone components were analyzed with gas chromatography-mass spectrometry by computer. The results showed that the peak of sex pheromone production and release occurred when the 3 days old female adults were treated in scotophase for 6 hours. However, the sex pheromone components were not found with the adsorption and SPME methods.