三种猪源性细胞外基质体内降解规律的实验研究

An in Vivo Study of the Degradative Regularity about Three Porcine-derived Extracellular Matrix

目的：研究本实验以小鼠腹壁缺损为模型,通过对三种猪源性细胞外基质（ECM）—猪脱细胞小肠粘膜下层（P-SIS）、猪脱细胞心包（P-PC）以及猪脱细胞真皮（P-ADM）,进行体内弹性纤维和葡糖氨基聚糖类（GAGs）的含量测定,然后通过三组材料中弹性纤维和GAGs在术后第4周、第8周时再生或降解的数量,来对比研究三种生物材料植入体内后的降解规律,从而为寻找性能优良的生物补片提供理论依据,为生物补片的临床应用以及避免术后并发症方面提供指导方向。方法：BALB/c小鼠60只,体重18～20 g,手术造成1 cm×1 cm的腹壁全层缺损,随机分为3组（n=20）,分别以等面积的P-ADM、P-PC以及P-SIS进行修复,于术后4周、8周进行取材评估。结果：术后P-SIS组的腹腔内粘连评分均低于同时期内的其余两组。术后4、8周比较：P-ADM组,GAGs含量减少（P〈0.01）；P-PC组,弹性纤维（P〈0.01）、GAGs（P〈0.05）含量均有所增加；P-SIS组,弹性纤维（P〈0.001）、GAGs（P〈0.05）含量均增加。结论：P-SIS材料中,弹性纤维和GAGs的再生状况优于其他两组,且P-SIS材料不易引起腹腔内粘连,故P-SIS能够更好的应用于组织缺损的治疗,P-SIS在组织工程学领域相对而言是更加理想的修复材料。

Objective： The purpose of this study is to research the degradative regularity about three porcine-derived extracellular matrix （ECM）： small intestine submucosa （P-SIS）, pericardium （P-PC） and acellular dermal matrix （P-ADM）, by quantitating the change of elastin, glycosaminoglycans （GAGs） in mouse model at 4 weeks and 8 weeks after surgery. Therefore, our study can provide the theory for searching an excellent biological mesh, and point the way for clinical application and avoiding postoperative complications of biological mesh. Methods： 60 BALB/c mouse, weighting 18 -20 g, Abdominal wall defects （1 cm × 1 cm ）were created by surgery and were repaired with the same area of P-SIS, P-ADM and P-PC, randomly divided into three groups （n=20）, The animals were sacrificed at 4 and 8 weeks after surgery. Results： The scores of abdominal adhesion in P-SIS group were lower than in the other two groups. In comparison between 4 weeks and 8 weeks after surgery, GAGs in P-ADM groups were decreased（P〈0.01）; elastin（P〈0.01 ）, GAGs（P〈0. 05） in P-PC groups were increased; elastin （P〈0.001）, GAGs （P〈0.05） in P-SIS groups were all increased. Conclusion： The regeneration of elastin and GAGs in P-SIS groups was better than in the other two groups, and P-SIS was good for anti-adhesion, relatively, so P-SIS could be applied in the treatment of tissue defect preferably, and it was an ideal material for tissue engineering application.