刺参酸性粘多糖对H22肝癌小鼠免疫功能的影响(英文)

Immune Effect of Stichopus Japonicus Acid Mucopolysaccharide on Hepatocarcinoma22-bearing Mouse

目的:刺参酸性粘多糖作为一种天然生物活性物质,具有较强抗肿瘤作用。本研究观察刺参酸性粘多糖对荷瘤小鼠免疫功能的影响,以探讨刺参酸性粘多糖的抗肿瘤作用机制。方法:皮下接种H22小鼠肝癌细胞,建立移植瘤小鼠模型。将50只荷瘤小鼠随机分为五组(阴性对照组、氟尿嘧啶组、SJAMP低剂量组、SJAMP中剂量组、SJAMP高剂量组),腹腔注射不同剂量刺参酸性粘多糖,每日一次,连续12天。眼球摘除取血后颈椎脱臼处死小鼠,计算抑瘤率和脏器指数,中性红法测定小鼠腹腔巨噬细胞吞噬功能,CCK-8法测定小鼠脾淋巴细胞增殖能力,ELISA法测定小鼠血清TNF-α水平。结果:SJAMP能够明显抑制肿瘤生长(P<0.05);与5-FU组相比,SJAMP干预组脾指数和胸腺指数明显升高(P<0.05),腹腔巨噬细胞吞噬能力和脾脏淋巴细胞增殖功能显著提高(P<0.05),TNF-α的血清含量显著减少(P<0.05)。结论:刺参酸性粘多糖通过促进免疫器官生长,增强机体的免疫功能,抑制小鼠H22肝癌生长。这为SJAMP的抗肿瘤作用研究提供了试验依据和理论基础。

Objective： As a natural biologically active substance, Stichopus japonicus acid mucopolysaccharide（SJAMP） has significant anti-tumor effect. The present study aimed to investigate the immune effect of SJAMP on Hepatocarcinoma22-bearing mouse.Methods： The mice were injected H22 cells into subcutaneous of the right armpit, then were randomly divided into model group, 5-FU group and SJAMP intervention groups（group A, B, C）. Three doses of SJAMP（6.25 mg/kg, 12.5 mg/kg, and 25 mg/kg administered once a day via intraperitoneally inject） were given to mice continuously for 12 days. Blood samples were drawn by excising eyeball and then all the mice were sacrificed by cervical dislocation. The spleens and thymuses were removed under sterile conditions, and the spleen indexes and thymus indexes were calculated. Neutral red method was used to detect macrophage phagocytosis. Spleen Lymphocytes proliferation capability was assayed by CCK-8 method and TNF-α was detected by ELISA method. Results： SJAMP treatment significantly inhibited Hepatocarcinoma22 by reducing the weight of tumor nodules. SJAMP administration also improved spleen indexes and thymus indexes, abdominal macrophage phagocytosis and spleen Lymphocytes proliferation activity. Furthermore, SJAMP groups showed a decrease in serum level of TNF-α. Conclusion： SJAMP effectively inhibits the growth of tumor through stimulating the immune organs proliferation and enhancing immunity ability of Hepatocarcinoma22-bearing mouse. The results provide an experimental and theoretical basis for the study of the anti-tumor effects of SJAMP.