摘要
目的观察缺氧_复氧对大鼠心室肌细胞的损伤及川芎嗪的保护作用。方法在缺氧_复氧条件下 ,测量对照组和不同浓度川芎嗪组对杆形心肌细胞百分比、心肌细胞内K +/Na+浓度比及Ca2 +浓度的影响。结果缺氧_复氧对大鼠心室肌细胞有损伤作用 ,浓度为4μmol·L -1 的川芎嗪对缺氧_复氧损伤细胞有保护作用 ,它能抑制损伤细胞挛缩 ,提高损伤细胞生存率 ,抑制心肌细胞内K +/Na +浓度比的降低、增加细胞内Ca2 +浓度 ,且剂量越大 ,保护作用越好 ,即川芎嗪对缺氧_复氧损伤细胞的保护呈剂量依赖性。结论川芎嗪对缺氧_复氧损伤的大鼠心肌细胞具有保护作用 。
Aim To observe anoxia_reoxygenation damage to ventricular myocytes and the protective effects of tetramethylpyrazine on the damage in rats. Methods The myocytes viability, intracellular K+/Na+, intracellular free calcium concentration were detected in different tetramethylpyrazine concentration groups and control group in the anoxia_reoxygenation damage condition. Results Anoxia_reoxygenation had done harm to the rat ventricular myocytes. Tetramethylpyrazine inhibited the myocyte contracture, increased the cell viability, inhibited the fall of intracellular K+/Na+,and increased intracellular free calcium concentration in the dose of 4 μmol·L-1 or more. And the more the dose is, the better the salutary effects are. Conclusions Tetramethylpyrazine has protective effect during anoxia_reoxygenation on the ventricular myocytes of rats.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2000年第1期18-20,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
湖北省"九五"攻关项目 №962P1101
