全反式维甲酸和阿糖胞苷对HL-60细胞端粒酶活性的影响

Effect of the all trans retinoic acid and Ara-c on the telomerase activity of HL-60 cells

目的探讨全反式维甲酸 (alltrans_retinoicacid,ATRA )和阿糖胞苷 (Ara_c)诱导人早幼粒细胞白血病细胞凋亡和分化的机制。方法采用PCR_ELISA方法观察ATRA和Ara_c对HL_60细胞端粒酶活性的影响。结果ATRA和Ara_c在诱导HL_60细胞凋亡和分化过程中均可使端粒酶活性减低。结论ATRA和Ara_c抑制HK_60细胞端粒酶的活性 。

Aim To investigate the possible mechanism of human promyelocytic leukemia cells differentiation and apoptosis induced by all trans_retinoic acid (ATRA) and Ara_c. Methods The effects of ATRA and Ara_c on the tolomerase activity of HL_60 cells were detected by PCR_ELISA. Results The tolomerase activity of HL_60 cells declined during the course of differentiation and apoptosis induced by ATRA and Ara_c. Conclusion These findings suggest that ATRA and Ara_c can inhibit tolomerase activity of HL_60 cells. It may be one of the important mechanisms of human promyelocytic leukemia cells differentiation and apoptosis induced by ATRA and Ara_c.