正交试验优选雷公藤提取工艺

Optimization of Extraction Process for Tripterygium wilfordii by Orthogonal Design

目的:优选雷公藤的提取工艺。方法:以雷公藤甲素和总二萜内酯提取量为指标,通过L9(34)正交试验考察提取时间、乙醇用量及体积分数对雷公藤提取工艺的影响。采用HPLC测定雷公藤甲素含量,流动相甲醇-水(45∶55),检测波长218 nm;利用UV测定总二萜内酯含量,检测波长550 nm。结果:乙醇体积分数对雷公藤甲素提取量具有显著性影响,其他2个因素则无显著性差异;各因素对总二萜内酯提取量均无显著性影响。最佳提取工艺为加8倍量95%乙醇回流提取3次,提取时间分别为2,1,1 h;雷公藤甲素、总二萜内酯提取量分别为4.20,86.64μg·g-1。结论:优选的提取工艺稳定可行,指标成分提取效率较高。

Objective： To optimize extraction process of Tripterygium wilfordii. Method： Taking extracting amounts of triptolide and total diterpene lactones as indexes, orthogonal test was adopted to optimize extraction process with extracting time, ethanol concentration and consumption as factors. HPLC was adopted to determine the content of triptolide with mobile phase of methanol-water （45： 55） and detection wavelength at 218 nm; UV was employed to determine the content of total diterpene lactones with detection wavelength at 550 nm. Result：Ethanol concentration had significant effect on extracting amount of triptolide, but the other two factors had no significant difference; three factors all had no significant effect on the content of total diterpene lactones. Optimum extraction condition were as follows： reflux extracted thrice with 8 times the amount of 95% ethanol for 2, 1, 1 h; extracting amounts of triptolide and total diterpene lactones were 4.20, 86.64 μg·g^-1, respectively. Conclusion： This optimized process was feasible and stable with high extracting efficiency of index components.