球旁注射高三尖杉酯碱在兔眼球组织的分布

Determination of homoharringtonine distribution in eyeball tissue ofrabbit by high performance liquid chromatography method

目的建立测定兔眼球组织中高三尖杉酯碱(HHT)的含量及其分布。方法以三尖杉酯碱(HT)为内标 ,荧光法检测 ;眼球旁注射HHT ,分离各眼球组织分别制匀浆、萃取后进行测定。结果HHT与HT的保留时间 (tR)分别为6.35min和4.70min ;在5～200μg·L-1 线性相关系数为0.994。平均加样回收率为 (93.17±4.31) %。眼球旁注射HHT2mg 后兔眼各组织含量按巩膜、角膜、视网膜、玻璃体、晶状体依次下降 ,并且巩膜组织内含量远高于其他组织 ,但消除速率却呈相反顺序。

Aim To observe homoharringtonine(HHT) distribution in eyeball tissue of rabbit. Methods Harringtonine(HT) was used as internal standard. The chromatography consisted of Kromasil C18 column with methanol 0.01mol·L-1 (60∶40,pH 2.5) as the mobile phase at the flow rate of 0.8ml·min-1. Results The chromatography retention time of HHT and HT were 6.35min and 4.70min. The standard curve was linear in the concentration range of 5～200 μg·L-1 in serum and the relation coefficient was 0.994. The recovery of spiked sample was(93.17±4.31)%. After injection of 2mg of HHT in side of rabbit eyeball, the contents in sclera, cornea, retina, vitreous body and crystalline lens were reduced progressively, but the eliminate rate were reduced in a proper order. Conclusion HHT can distribute to every eyeball tissue when it was injected in side of rabbit eyeball.