红花龙胆高效液相指纹图谱

HPLC Fingerprint Chromatograms of Gentiana rhodantha

目的:建立红花龙胆药材的HPLC指纹图谱的测定方法。方法:采用以Diamonsi L C18色谱柱(4.6 mm×200mm,5μm),流动相乙腈(A)-0.05%磷酸水溶液(B)梯度洗脱,流速0.8 mL·min-1,检测波长245 nm,柱温25℃。结果:建立了红花龙胆药材HPLC指纹图谱,标定了40个共有峰,以芒果苷为参照峰,12批红花龙胆药材的相似度均>0.97。结论:该法重复性好,专属性强,可为红花龙胆药材质量控制提供科学依据。

Objective： To established the HPLC fingerprint chromatograms of Gentiana rhodantha. Method： The HPLC analysis was performed on a Diamonsil C18 （4.6 mm×200 mm, 5 μm） column with isocratie elution of aeetonitrile-0.05% phosphoric acid at a flow rate of 0. 8 mL ·min^-1. The detection wavelength was set at 245 nm, and the column temperature was 30 ℃. Result： The HPLC fingerprint chromatograms of G. rhodantha has been established, there were 40 common peaks, the similarities of the fingerprint chromatograms were calculated over 0.97 between 12 batches of G. rhodantha samples with mangiferin as reference substance. Conclusion： The method is repeatable, exclusive and can be used quality control of G. rhodantha.