摘要
目的:对竹叶柴胡和北柴胡进行挥发性成分的比较和分析,指出竹叶柴胡和北柴胡的差异性和共同性。方法:利用固相微萃取技术(SPME)提取,采用气质联用(GC-MS)进行分离和鉴定。结果:共鉴定出竹叶柴胡48个成分,北柴胡21个成分,其中有10个化合物为共有峰,相对质量分数最高的均为n-十六烷酸。结论:SPME-GC-MS能全面快速分析柴胡挥发油成分,并作为柴胡药材质量评估和基源鉴定的科学手段。
Objective: To find the difference and the identity through analyzing the essential oil of Bupleurum marginatum and B. chinese. Method: The essential oil components of B. marginatum and B. chinese were extracted by solid phase micro extraction (SPME) and analyzed by GC-MS. Result: Forty-eight compounds were identified from B. marginatum, and 21 from B. Chinese. Ten common compounds were found and n-hexadecanoic acid was the main compound of highest relative content in both of B. marginatum and B. chinense. Conclusion: The information about the constituents of essential oil can be obtained entirely and quickly by SPME- GC-MS which is the scientific method of quality assessment and origin identification.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第14期104-108,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家星火计划项目(2010GA810056)