Toll样受体-4与血管内皮生长因子在肾癌组织中的表达及临床意义

Expressions and significance of TLR4 and vascular endothelial growth factor in clear cell renal cell carcinomas

目的观察肾透明细胞癌中Toll样受体4(TLR4)和血管内皮生长因子(VEGF)的表达及其临床意义。方法应用免疫组化方法检测72例肾透明细胞癌患者的肿瘤组织、瘤旁肾组织以及远离肿瘤的正常肾组织中TLR4和VEGF的表达,并结合肾癌患者的临床病理特征进行比较。结果 TLR4在肾癌组织中的表达明显高于癌旁肾组织和正常肾组织(P<0.05);其在肿瘤中的表达强度与TNM分期以及是否合并淋巴结转移有关。VEGF在肾癌组织中的表达高于瘤旁肾组织和正常肾组织(P<0.05);其在肿瘤中的表达强度也与TNM分期以及是否合并淋巴结转移有关。TLR4的表达与VEGF的表达呈正相关(r=0.268,P<0.05)。结论 TLR4和VEGF与肾透明细胞癌的临床病理特征存在一定的联系,在肾透明细胞癌发生、发展过程中起重要作用。

【Objective】To investigate the protein expression of TLR4 and vascular endothelial growth factor（VEGF） in clear cell renal cell carcinomas（RCC） and concomitant renal tissues.【Methods】Tumor tissues and corresponding peritumoral and macroscopically normal kidney tissues were collected from 72 patients with RCC. The expression of TLR4 and VEGF was evaluated immuohistochemically in relation to clinic opathlogical characteristics.【Results】TLR4 protein expression was significantly higher in tumoral tissues than in peritumoral and normal tissues（P〈0.05）. TLR4 expression intensity was associated with the TNM stage and lymph node metastasis. VEGF protein expression was significantly higher in tumoral tissues than in peritumoral and normal tissues（P〈0.05）. There were significant differences in VEGF expression in relation to TNM stage and lymph node metastasis. TLR4 expression correlated positive with VEGF expression（r =0.268, P〈0.05）.【Conclusions】There are associations between the expression of TLR4 and VEGF and the clinic opathlogical factors of clear cell RCC. TLR4 and VEGF may play a vital role in tumor growth and progression.