青花菜与萝卜属间杂种的表型和分子鉴定

Identification of Intergeneric Hybrids between Broccoli( Brassica oleracea L. var italica Plenck. ) and Radish( Raphanus sativus L. ) by Morphological Traits and SSR Marker

应用形态学、细胞学和分子标记的方法对通过胚挽救获得的青花菜与萝卜属间杂种植株进行了鉴定。结果表明:杂种植株的生长势明显强于父本和母本,大部分形态性状超出了父、母本的范围,部分性状介于父母本之间或偏向于父本或母本一方。表型和细胞学观察显示杂种植株具有类似母本的雄性不育特性。SSR分子标记分析表明,杂种植株包含了双亲的遗传信息。可见,远缘杂种是双亲间的真杂种。远缘杂交种的获得与初步鉴定为促进萝卜属和芸薹属的基因交流提供了桥梁种质,为进一步创制萝卜或芸薹属作物新种质提供了基础材料。

The intergeneric hybrids of broccoli and radish were obtained by embryo rescue and identified by morphological characterization,cytological observation,and molecular marker techniques. The results showed that the hybrids showed stronger growth vigor compared with their parents. Most phenotypic characters of the hybrids were beyond two parents. Cytological observation revealed that the hybrid plants possessed male sterility characteristic as female parent broccoli. According to SSR molecular marker analysis,the hybrids contained genetic information of both parents,which proved that hybrids were real hybrids. The creation and identification of the hybrids could provide basic materials for producing new radish germplasm and useful bridge for gene communication between Brassica and Raphanus.