摘要
为探讨不同基因型割手密无性系间的亲缘关系,采用酶解去壁低渗法对不同基因型的割手密材料进行核型分析。10份供试材料的绝大多数染色体为中部着丝点(m)染色体,少数为近中部着丝点(sm)染色体及正中部着丝点(M)染色体,部分材料中还具有端部着丝点(T)染色体及近端部着丝点区染色体(t);依据分析结果总结了参试材料的核型公式;参试材料中有2份为1B核型、7份为2B核型、1份为2C核型。参试材料间的核型均存在差异且不对称。
To study the relationship between different Saccharum spontaneum L. genotype clones,karyotype analysis of these clones were performed by using the method of wall degradation and low osmosis. The results showed that most of the chromosomes were median region( m) types,only a few were sub-median region( sm),median point( M),terminal point( T),and terminal region( t) types in all of the materials used for study. The karyotype formulas of each accession were summarized based on the datum. The karyotype of 2 accessions exhibited 1B type,7 accessions for 2B,and 1 accession for 2C. All of the materials showed karyotypic difference and asymmetry types.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第4期912-917,共6页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31260348)
云南省现代农业甘蔗产业技术体系(云农发科[2009]12号)
云南农业大学高原山地作物可持续生产系统研究省创新团队"高原山地特色作物种质创新及利用"(云科人发[2012]18号)
云南农业大学博士科研基金项目(A2002181)
云南省应用基础研究面上项目(2012FB150
2013FB037)
关键词
甘蔗
割手密
无性系
核型
Sugarcane
Saccharum spontaneum
clone
karyotype