不同基因型割手密无性系的核型分析

Karyotype Analysis of Different Saccharum spontaneum Genotype Clones

为探讨不同基因型割手密无性系间的亲缘关系,采用酶解去壁低渗法对不同基因型的割手密材料进行核型分析。10份供试材料的绝大多数染色体为中部着丝点(m)染色体,少数为近中部着丝点(sm)染色体及正中部着丝点(M)染色体,部分材料中还具有端部着丝点(T)染色体及近端部着丝点区染色体(t);依据分析结果总结了参试材料的核型公式;参试材料中有2份为1B核型、7份为2B核型、1份为2C核型。参试材料间的核型均存在差异且不对称。

To study the relationship between different Saccharum spontaneum L. genotype clones,karyotype analysis of these clones were performed by using the method of wall degradation and low osmosis. The results showed that most of the chromosomes were median region（ m） types,only a few were sub-median region（ sm）,median point（ M）,terminal point（ T）,and terminal region（ t） types in all of the materials used for study. The karyotype formulas of each accession were summarized based on the datum. The karyotype of 2 accessions exhibited 1B type,7 accessions for 2B,and 1 accession for 2C. All of the materials showed karyotypic difference and asymmetry types.