斑马鱼对3种氯酚的富集作用及其SOD酶活性应激反应研究

Study on Bioconcentration of Three Chlorophenols in Zebrafish and SOD Activity Stress Action

为了研究对氯酚(4-CP)、2,4-二氯酚(2,4-DCP)、2,4,6-三氯酚(2,4,6-TCP)对斑马鱼的生理毒性作用,以7 d富集系数(KBCF)和超氧化物歧化酶(SOD)活性为评价指标,采用半静态试验法对斑马鱼进行驯养和试验,测定斑马鱼对3种氯酚的7 d KBCF,同时测定不同浓度条件下,暴露1 d和7 d,斑马鱼体内SOD酶活性。结果表明,4-CP、2,4-DCP和2,4,6-TCP的7 d KBCF分别为33.65±5.29、65.54±10.49和128.05±39.83,富集能力表现为4-CP(无富集)<2,4-DCP(轻度富集)<2,4,6-TCP(轻度富集);斑马鱼对氯代酚类物质的富集性与氯酚类物质氯原子取代个数正相关。3种氯酚胁迫斑马鱼结果表明,暴露1 d表现为诱导-抑制效应,暴露7 d表现为抑制效应,酶活性变化显著。

To study the physio-toxicity effect of 4-chlorophenol, 2,4-dichlorophenol and 2,4,6-trichlorphenol on zebrafish, the evaluation indicator of 7 d KBCF and SOD activity with different exposure concentrations were determined using zebrafish by semi-static test.The results showed that the 7 d KBCF of 4-CP, 2, 4-DCP and 2,4,6-TCP to zebrafish were 33.65 ±5.29, 65.54 ±10.49 and 128.05 ±39.83, respectively, and the bioconcentration factor followed the order of 4-CP 〈2, 4-DCP 〈2, 4, 6-TCP. The bioconcentration of the chlorophenols to zebrafish and the numbers of chloro derivatives had positive correlation .The results of stress action test of the chlorophenols to zebrafish showed that the effect at 1 d was induction-inhibition and at 7 d the effect was inhibition , indicating significant variation of SOD activity .