分子开关蛋白激酶在不明原因复发性流产患者蜕膜组织中的表达

Expression of SGK in uterine decidua of patients with unexplained recurrent spontaneous abortion

目的 观察不明原因复发性流产（URSA）患者蜕膜组织中蛋白激酶（SGK）的表达及其在介导细胞凋亡中的相关性.方法 采用免疫组织化学SP法检测URSA患者组50例（流产组）和30例人工流产（对照组）蜕膜组织中SGK的表达情况,应用TUNEL法检测两组细胞凋亡指数.结果 流产组中SGK的阳性表达率（24.0％）显著低于对照组（93.3％）,差异有统计学意义（χ2=6.78,P ＜0.05）.流产组的平均细胞凋亡指数[（8.19±3.58）％]高于对照组[（2.87±1.07）％],差异有统计学意义（t=7.94,P＜0.05）.结论 蜕膜组织细胞凋亡明显增加、SGK表达降低可能在URSA中具有重要作用.

Objective To observe the expression of SGK in decidua in patients with unexplained recurrent spontaneous abortion（URSA） and to investigate its role in the course of apoptosis.Methods The expression of SGK was detected by immunohistochemistry in decidua of URSA（abortion group,n =50） and normal first trimester pregnant women（control group,n =30）.The apoptotic index was detected by TUNEL.Results Compared with the control group,the positive expression of SGK decreased significantly in the abortion group,and the difference was statistically significant（χ2 =6.78,P 〈 0.05）.The average apoptotic index of the abortion group was （8.19 ± 3.58） %,which was significantly higher than （2.87 ±1.07）% of the control group（t =7.94,P〈0.05）.Conclusion Intensive apoptosis,the decreasing positive expression rate of SGK in decidua cells may play critical roles in URSA.