CD4＋CD25＋调节性T细胞在自身免疫性溶血性贫血患者中的变化及意义

Change and significance of CD4＋CD25＋ regulatory T cells in patients with autoimmune hemolytic anemia

目的 探讨CD4＋CD25＋Foxp3＋调节性T细胞（Treg）在自身免疫性溶血性贫血（AIHA）患者中的变化.方法 应用流式细胞术检测AIHA患者治疗前后外周血Treg的数量变化.结果 AIHA患者治疗前外周血中Treg细胞比例为（1.32±0.87）％,明显低于对照组（3.08±0.96）％（t=-5.37,P＜0.01）.治疗后Treg细胞比例为（4.96±1.13）％,明显高于治疗前（t=-16.94,P＜0.01）,且高于对照组（t=-4.96,P＜0.01）.结论 AIHA患者中Treg细胞数量下降,糖皮质激素通过上调Treg细胞数量可以控制自身免疫性溶血性贫血.

Objective To investigate the change of regulatory T-cells （Treg） before and after therapy in patients with autoimmune hemolytic anemia （AIHA）,and to study the role of Treg in AIHA.Methods Treg cells numbers was measured by flow cytometry.Results Before treatment,Treg cells in AIHA patients was （1.32 ± 0.87） %,which was significantly lower than （3.08 ± 0.96） % in the controls （t =-5.37,P 〈 0.01）.After treatment,Treg cells in AIHA patients was significantly increased [（4.96 ± 1.13）%] （t =-16.94,P 〈0.01）.Conclusion Treg cells decreased in AIHA patients.Glucocorticoid might play a role in AIHA treatment by up-regulating Treg cells number.