萎胃颗粒对慢性萎缩性胃炎大鼠胃黏膜Ubiquitin表达的影响

Effects of Weiwei Granule on Ubiquitin Expressions in Gastric Mucosa of Chronic Atrophic Gastritis Rats

目的:探讨萎胃颗粒对慢性萎缩性胃炎大鼠胃黏膜Ubiquitin表达的影响,进一步明确其作用机制。方法:通过综合法复制大鼠慢性萎缩性胃炎模型,造模成功后分为正常组、模型组及萎胃颗粒低、中、高剂量组,从而观察萎胃颗粒对慢性萎缩性胃大鼠胃黏膜Ubiquitin表达的影响。结果:Ubiquitin蛋白OD值在正常组为(2.17±0.62),模型组(3.39±0.58),萎胃颗粒低剂量组(2.92±0.65),中剂量组(2.67±0.63),高剂量组(2.34±0.56)。与模型组比较:萎胃颗粒各治疗组OD值均有明显下降(P<0.01),且这种作用呈剂量依赖性,即低剂量组>中剂量组>高剂量组,3组治疗组比较差异具有统计学意义(P<0.01)。结论:萎胃颗粒可能通过抑制CAG大鼠胃黏膜Ubiquitin的表达,减少IκBα的降解,进而抑制NF-κB的活化,降低了胃黏膜炎症程度。

Objective：To explore the effects of Weiwei granule on Ubiquitin expressions in gastric mucosa of chronic atrophic gastritis（CAG） rats and further investigate the mechanisms.Methods：With the comprehensive methods,the rat models of CAG were established and assigned into normal group,modeling group and Weiwei granule groups with low,middle and high doses,and the effects of Weiwei granule on Ubiquitin expressions in gastric mucosa of CAG rats were observed.Results：The OD values of Ubiquitin protein were（2.17 ± 0.62） in the normal group,（3.39 ± 0.58） in the modeling group,（2.92 ± 0.65） in the low-dose Weiwei granule group,（2.67 ± 0.63） in the middle-dose Weiwei granule group and（2.34 ± 0.56） in the high-dose Weiwei granule group.Compared with that in the modeling group,the OD values in all the Weiwei granule groups were obviously decreased（P〈0.01）,which had the tendency of dosage-dependency,namely,low-dose group the highest and high-dose group the lowest,with significant difference among the three groups（P〈0.01）.Conclusion：Weiwei granule could inhibit Ubiquitin expression in gastric mucosa of CAG rats and reduce the degradation of IκBα so as to inhibit the activation of NF-κB and reduce the inflammation of gastric mucosa.