酿酒酵母发酵条件的研究

Fermentation conditions of Saccharomyces cerevisiae

以实验室前期筛选的培养基为发酵培养基,通过单因素试验和正交试验,得到酿酒酵母的最佳培养条件为温度26℃、转速150 r/min、初始pH 5.5、培养时间36 h、接种量4%、装液量20 mL/100 mL,在此优化条件下进行发酵,其OD560nm为1.370,菌体密度为1.54×108个/mL,菌体干质量可以达到18.48g/L,比初始条件下菌体干质量提高了将近10%。

Using the medium screened in the early stage, the optimal fermentation conditions of Saccharomyces cerevisiae were optimized by single factor experiment and orthogonal experiment. The optimized culture conditions were temperature 26 ℃, rotate speed 150 r/min, initial pH 5.5, incubation time 36 h, inoculum 4%, fluid volume 20 ml/100 ml. Under the optimal condition, the OD560nmwas 1.370, yeast density was 1.54×108CFU/ml,the dry cell weight reached 18.48 g/L, which was nearly 10% more than the initial condition.