中药软肝宁对实验性肝纤维化大鼠TGFβ_1、Smad2/3表达的影响

Effect of traditional Chinese medicine Ruan Gan Ning on the expression of TGFβ_1 and Smad2/3 in the experimental rat hepatic fibrosis

目的观察四氯化碳(CCl4)诱导大鼠肝纤维化后应用软肝宁的疗效及作用机制。方法用40%CCl4对Wistar大鼠造模8周,将50只分为中药预防组、中药治疗组、模型对照组及正常对照组,用软肝宁于造模第1周及第4周开始灌胃。运用免疫组织化学(S-P)技术检测转化生长因子β1(TGFβ1)、Smad2/3在肝内表达,碱水解法检测肝组织羟脯胺酸(Hyp)含量,酶联免疫法检测肝组织内透明质酸(HA)的含量。结果经软肝宁防治后大鼠肝组织内TGFβ1、Smad2/3表达减少,透明质酸、羟脯胺酸水平降低。结论复方中药制剂软肝宁能有效地逆转四氯化碳诱导肝纤维化大鼠的纤维化,其机理可能是抑制肝内TGFβ1、Smad2/3表达。

Objective To observe the therapeutic effect and mechanism of Ruan Gan Ning on the rat hepatic fibrosis induced by carbon tetrachloride（CCIAL）. Methods 50 Wistar rats were divided into normal control, model control, TCM prevention and TCM therapy groups. At the first and forth week of model-making, the rats were given Ruan Gan Ning through intragastric administration. The expression of TGF β1 and Smad2/3 in the liver tissue was detected with immunohistochemistry（S-P） and the content of Hyp with alkaline hydrolysis, the content of hyaluronic acid（HA） with euzymelinked immunosorbent assay. Results After the application of Ruan Gan Ning the expression of TGF β1 Smad2/3 and the level of HA and Hyp decreased in the experimental rat hepatic fibrosis. Conclusion Ruan Gan Ning can relieve the rat hepatic fibrosis induced by CCIA and the mechanism of action may be its inhibition of expression of TGF β1, Smad2/3 in the liver tissue.