亮叶杨桐叶乙酸乙酯部位抗炎镇痛作用的研究

Study on the anti-inflammation and analgesic effect of ethyl acetate extract from adinandra nitida leaves

目的观察亮叶杨桐叶乙酸乙酯部位(LY)的抗炎和镇痛作用,为进一步开发亮叶杨桐叶的药用价值提供参考。方法以巴豆油致小鼠耳肿胀法、冰醋酸致小鼠腹腔毛细血管通透性亢进法和大鼠棉球肉芽肿慢性炎症法,观察LY的抗炎作用;以小鼠热板法、大鼠足趾机械刺痛法和大鼠尾部辐射热刺激法,观察LY的镇痛作用。结果与模型组比较,LY(0.42 g/kg)能显著抑制化学刺激引起的小鼠耳肿胀(P<0.05)和小鼠腹腔毛细血管通透性增高(P<0.01),同时能显著抑制大鼠棉球肉芽组织增生(P<0.01);与模型组比较,LY(0.42 g/kg)可明显抑制热刺激(P<0.05)和机械刺激引起的疼痛(P<0.05),对尾部辐射热刺激引起的疼痛无明显抑制作用(P>0.05)。结论 LY对急慢性炎症和高位中枢参与的疼痛有良好的抑制作用。

Objective To study the anti-inflammatory and analgesic effect of ethylacetate extract from Adinandra Nitida leaves（ LY）,and provide a reference for the further development of medicinal value. Method The acute inflammatory models of the auricle swelling in mice induced by croton oil and the increase of capillary permeability in mice induced by acetic acid,and the chronic inflammatory model of the granuloma in rat induced by cotton ball were used to evaluate the anti-inflammatory effect of LY.The hot-plate test（ 55℃） in mice,mechanical stimulation and radiant heat flick test in rat were used to evaluate the analgesic effect of LY. Result Compared with model group,LY（ 0. 42 g /kg） was significantly inhibited the swelling of mice auricle（ P〈 0. 05）and the permeability of the blood capillary in abdominal cavity of mice（ P〈 0. 05）,and reduced the weight of rat cotton pellet granuloma（ P〈 0. 01）. Compared with model group,LY（ 0. 42 g /kg） was significantly inhibited pain reaction induced by hot-plate（ P〈 0.05） and mechanical stimulation（ P〈 0. 05）,but had no obvious inhibition on pain reaction induced by tail radiant heat（ P〈 0.05）. Conclusions LY has strong anti-inflammatory and analgesic activities.