STAT3、Cyclin D1在大鼠肝癌组织中的表达变化及意义

Expression changes and significance of STAT3 and cyclin D1 in hepatocellular carcinoma tissues of rats

目的观察信号转导和转录活化因子3(STAT3)和细胞周期素D1(Cyclin D1)在大鼠肝癌组织中的表达变化,并探讨其意义。方法将72只大鼠随机分为模型组和对照组各36只,模型组自由饮用0.1 mg/mL的二乙基亚硝胺(DEN)溶液,对照组饮用等量灭菌蒸馏水,连续饮用20周。处死大鼠取癌组织,用RT-PCR法检测STAT3、Cyclin D1 mRNA,Western blot法和免疫组化法检测STAT3、Cyclin D1蛋白。结果对照组STAT3、Cyclin D1mRNA表达水平分别为0.32±0.12、0.18±0.05,模型组分别为0.72±0.25、0.57±0.15,P均<0.05。Western blot法检测模型组STAT3、Cyclin D1蛋白表达量分别为0.58±0.25、0.65±0.14,对照组分别为0.32±0.19、0.41±0.15,P均<0.05。免疫组化法检测模型组STAT3、Cyclin D1蛋白IOD分别为2 373.87±258.79、668.44±63.10,对照组分别为487.81±55.31和138.86±31.50,P均<0.05。结论 STAT3、Cyclin D1在肝癌大鼠癌组织中表达上调,二者可促进肝癌的发生和发展。

Objective To observe the expression changes of signal transducer and activator of transcription 3（ STAT3） and cyclin D1 in hepatocellular carcinoma tissues of rats and to investigate its significance. Methods Seventytwo rats were randomly divided into the model group and control group,36 rats in each group. The model group received0. 1 mg /mL diethylnitrosamine（ DEN） freely,and the control group was given the same volume of sterile distilled water for20 weeks. The STAT3 and cyclin D1 mRNA was detected by using RT-PCR,Western blotting and immunohistochemistry were used to detect the STAT3 and cyclin D1 protein expression. Results In control group,the mRNA expression of STAT3 and cyclin D1 were respectively 0. 32 ± 0. 12 and 0. 18 ± 0. 05,in the model group respectively 0. 72 ± 0. 25 and0. 57 ± 0. 15,all P〈 0. 05; in the control group,the protein expression levels of STAT3 and cyclin D1 were respectively0. 32 ± 0. 19 and 0. 41 ± 0. 15,while in the model group,they were respectively 0. 58 ± 0. 25 and 0. 65 ± 0. 14,all P〈 0. 05; immunohistochemical results showed that the IOD of STAT3 and cyclin D1 in the model group was respectively2 373. 87 ± 258. 79 and 668. 44 ± 63. 10,in control group,it was respectively 487. 81 ± 55. 31 and 138. 86 ± 31. 50,all P〈 0. 05. Conclusion The expression of STAT3 and cyclin D1 in the hepatocellular carcinoma tissues of rats is up-regulated,and both of them can promote the occurrence and development of hepatocellular carcinoma.