草豆蔻中一个新的二氢黄酮及其对PC12细胞的神经保护作用(英文)

A new flavonone from seeds of Alpinia katsumadai and its neuroprotective effect on PC12 cells

该文对草豆蔻Alpinia katsumadai中的黄酮类化学成分进行了研究。通过硅胶,Sephadex LH-20等柱色谱和制备液相等多种色谱方法,从草豆蔻95%乙醇提取物中分离得到7个黄酮类化合物。根据理化性质和波谱数据分别鉴定为(2R,3S)-pinobanksin-3-cinnamate(1)、乔松素(2)、短叶松素(3)、3-O-乙酰基短叶松素(4)、高良姜素(5)、华良姜素(6)、山柰酚-3-甲醚(7)。其中化合物1为新化合物,其他6个为已知化合物。药理活性研究表明,化合物1对皮质酮损伤的PC12细胞有很强的保护作用,其机制可能是通过清除细胞内活性氧发挥效果。

A new flavonone,named as（ 2R,3S）-pinobanksin-3-cinnamate（ 1）,together with six known compounds,pinocembrin（ 2）,pinobanksin（ 3）,3-O-acetylpinobanksin（ 4）,galangin（ 5）,kumatakenin（ 6）,and 3-methylkaempferol（ 7）,were isolated from a 95% ethanol extract of seeds of Alpinia katsumadai through a combination of various chromatographic techniques,including silica gel and Sephadex LH-20. The structure of compound 1 was elucidated by spectroscopic data analysis. Compound 1 exhibits a potent neuroprotective effect against the corticosterone-damaged PC12 cells,which may be underlying the effect by scavenging intracellular ROS.