摘要
研究接骨木Sambucus williamsii茎枝中的木脂素类化学成分,并讨论它们对大鼠骨肉瘤细胞UMR106增殖的影响。该研究采用D101大孔吸附树脂,硅胶,ODS,Sephadex LH-20,Toyopearl HW-40和RP-HPLC等色谱分离手段对接骨木茎枝60%乙醇提取物进行分离纯化,并运用波谱方法对所分离的化合物进行结构鉴定。从中分离鉴定了6个木脂素类化合物和1个酚酸类化合物,经波谱解析鉴定为threo-guaiacylglycerol-β-O-4’-conifery ether(1),lirioresinol A(2),1-hydroxypinoresinol(3),5-甲氧基蛇菰宁(4),蛇菰宁(5),5-methoxy-trans-dihydrodehydrodiconiferyl alcohol(6)和对羟基苯甲醛(7),化合物3~7首次从该属植物中分离得到。对所有分离得到的化合物进行了类成骨UMR106细胞增殖活性的测定,化合物1~7均能一定程度上促进UMR106细胞的增殖。
The present study aims to investigate the lignan constituents from Sambucus williamsii and their proliferation effects on osteoblast-like UMR106 cells. Seven compounds were isolated and purified by macroporous resin D101,silica gel,Sephadex LH-20,Toyopearl HW-40,ODS column chromatographies and Preparative HPLC( C-18). Their structures were elucidated by spectroscopic methods as threo-guaiacylglycerol-β-O-4'-conifery ether( 1),lirioresinol A( 2),1-hydroxypinoresinol( 3),5-methoxybalanophonin( 4),balanophonin( 5),5-methoxy-trans-dihydrodehydrodiconiferyl alcohol( 6),and p-hydroxybenzaldehyde( 7). Compounds 3-7were obtained from this genus for the first time. The proliferation effects of all isolated compounds on osteoblast-like UMR106 cells were determined. Compounds 1-7( 1 × 10-12-1 × 10-7mol·L-1) increased UMR106 cell proliferation to some extent.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第14期2684-2688,共5页
China Journal of Chinese Materia Medica