接骨木中的木脂素类化学成分及其对UMR106细胞增殖作用的影响

Lignans isolated from stems of Sambucus williamsii and their proliferation effects on UMR106 cells

研究接骨木Sambucus williamsii茎枝中的木脂素类化学成分,并讨论它们对大鼠骨肉瘤细胞UMR106增殖的影响。该研究采用D101大孔吸附树脂,硅胶,ODS,Sephadex LH-20,Toyopearl HW-40和RP-HPLC等色谱分离手段对接骨木茎枝60%乙醇提取物进行分离纯化,并运用波谱方法对所分离的化合物进行结构鉴定。从中分离鉴定了6个木脂素类化合物和1个酚酸类化合物,经波谱解析鉴定为threo-guaiacylglycerol-β-O-4’-conifery ether（1）,lirioresinol A（2）,1-hydroxypinoresinol（3）,5-甲氧基蛇菰宁（4）,蛇菰宁（5）,5-methoxy-trans-dihydrodehydrodiconiferyl alcohol（6）和对羟基苯甲醛（7）,化合物3～7首次从该属植物中分离得到。对所有分离得到的化合物进行了类成骨UMR106细胞增殖活性的测定,化合物1～7均能一定程度上促进UMR106细胞的增殖。

The present study aims to investigate the lignan constituents from Sambucus williamsii and their proliferation effects on osteoblast-like UMR106 cells. Seven compounds were isolated and purified by macroporous resin D101,silica gel,Sephadex LH-20,Toyopearl HW-40,ODS column chromatographies and Preparative HPLC（ C-18）. Their structures were elucidated by spectroscopic methods as threo-guaiacylglycerol-β-O-4＇-conifery ether（ 1）,lirioresinol A（ 2）,1-hydroxypinoresinol（ 3）,5-methoxybalanophonin（ 4）,balanophonin（ 5）,5-methoxy-trans-dihydrodehydrodiconiferyl alcohol（ 6）,and p-hydroxybenzaldehyde（ 7）. Compounds 3-7were obtained from this genus for the first time. The proliferation effects of all isolated compounds on osteoblast-like UMR106 cells were determined. Compounds 1-7（ 1 × 10-12-1 × 10-7mol·L-1） increased UMR106 cell proliferation to some extent.