大八角根化学成分研究

Chemical constituents from roots of Illicium majus

采用硅胶、Sephadex LH-20、ODS及制备型HPLC等多种色谱技术,从大八角根中分离得到10个化合物,根据理化性质及波谱数据（MS,1H,13C-NMR）分析分别鉴定为cycloparviflorolide（1）,cycloparvifloralone（2）,tashironin（3）,tashironin A（4）,anislactone A（5）,anislactone B（6）,pseudomajucin（7）,syringaldehyde（8）,methyl-4-hydroxy-3,5-dimethoxybenzoate（9）和（E）-3-methoxy-4,5-methylenedioxycinnamic alchol（10）。其中化合物1～4,8～10为首次从该植物中分离得到。在1.0×10-5mol·L-1下,在LPS刺激小鼠小胶质细胞释放NO抑制模型上,化合物5,6抑制率分别为75.31%,53.7%。

Ten compounds,including seven sesquiterpenes,two phenols and one phenylpropanoid,were isolated from the roots of Illicium majus by means of silica gel,ODS,Sephadex LH-20,and preparative HPLC. On analysis of MS and NMR spectroscopic data,their structures were established as cycloparviflorolide（ 1）,cycloparvifloralone（ 2）,tashironin（ 3）,tashironin A（ 4）,anislactone A（ 5）,anislactone B（ 6）,pseudomajucin（ 7）,syringaldehyde（ 8）,methyl-4-hydroxy-3,5-dimethoxybenzoate（ 9）,and（ E）-3-methoxy-4,5-methylenedioxycinnamic alchol（ 10）,respectively. Compounds 1-4 and 8-10 were first isolated from this plant. In the in vitro assays,at a concentration of 1. 0 × 10-5mol·L-1,compounds 5 and 6 were active against LPS induced NO production in microglia with a inhibition rate of 75. 31% and 53. 7%,respectively.