基于硫酸-蒽酮法对不同产地藏药线叶龙胆多糖的含量测定

Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method

研究线叶龙胆药材中多糖含量的测定方法,分析不同产地线叶龙胆药材中多糖的含量。结果表明,采用硫酸-蒽酮法,操作简便,结果准确;样品经硫酸-蒽酮显色后于620 nm处测定吸收度,在此波长处溶液的吸收度与葡萄糖含量呈良好的线性关系;线性范围0.01～0.07 g·L-1（r=0.996 7）,加样回收率99.41%,RSD 2.0%;综合考虑实验条件,确定料液比为1∶60、提取时间为50 min、乙醇浓度为4倍量（体积分数80%）。测得产自甘肃夏河的线叶龙胆中多糖质量分数为0.743%,含量最高,为线叶龙胆多糖的进一步研究奠定良好的基础。

Study a method for the detemination of the content of polysaccharides in Gentiana farreri,and analysis of the content of polysaccharides from different producing areas. The results showed that using the anthrone-sulfuric acid method,simple operation,accurate result. Sample was measured at 620 nm absorbance after anthrone-sulfuric acid color,at this wavelength,solution absorption and glucose showed a good linear relationship; The linearity was in the range of 0. 01-0. 07 g·L-1（ r =0.996 7）,The recovery rate was 99. 41%,with RSD of 2. 0%. Considering the experimental conditions,to determine the solid-liquid ratio 1∶ 60,extracting time50 min,concentration of ethanol 80%. The mass fraction of polysaccharides was the highest to reached 0. 743% in G. farreri from Gansu Xiahe. This experiment has laid a good foundation for further study on G. farreri.