大豆抗胞囊线虫的分子标记研究

Molecular Marker Assisted Selection on Soybean Resistant to Soybean Cyst Nematode

为了在分子水平验证大豆材料的抗病性,以对大豆胞囊线虫3号生理小种表现为抗性和感性的大豆为试材,利用已经报道的与SCN抗病基因连锁的SSR标记引物,对供试的大豆材料进行指纹图谱鉴定。结果表明:引物Satt130无扩增结果,Satt301没有多态性,Satt309和Satt082表现出多态性。聚类分析结果表明,所有供试材料被分成3大类,其中在合丰25×抗线4号正反交组合中,抗病亲本抗线4号与(抗线4号×合丰25)F2、(合丰25×抗线4号)F3抗病后代抗-221、抗-131聚为一类;感病亲本合丰25与感病材料(合丰25×抗线4号)F2感-121被聚为一类,在这个区域将感病材料分开。而其它后代材料则聚为一类,并没有将抗感材料区分开。

In order to verify the soybean resistance to SCN at the molecular level,taking resistance soybean and susceptible soybean to SCN as materials,and SSR markers linked to the resistance genes to SCN were used to identify the fingerprint of the selected material of soybean.The results indicated that there was no amplification result on Satt130and no polymorphism on Satt301,but polymorphism showed on Satt309and Satt082.The clustering analysis showed that materials were separated into 3classes,the Hefeng 25×Kangxian 4reciprocal cross,Kangxian 4and resistant offspring 7,8were in the same class,susceptible parent Hefeng 25and susceptible material 3were in another class,so the materials could be separated into resistance and susceptible.Other materials in the progeny were clustered in one class and did not separate the susceptible and resistant materials.