针刺捻转手法对脑出血家兔磁共振灌注加权成像的影响

Effect of twirling and rotating acupuncture manipulation on acute intracerebral hemorrhage rabbits studied by PWI

目的:观察针刺捻转平补平泻法对脑出血家兔血肿周围脑组织局部脑血流量的影响,探讨针刺手法在针刺治疗效应中的作用。方法:40只雄性新西兰兔随机分为正常组、模型组、留针组和手法组,每组各10只。自体血二次注血法复制急性脑出血模型。留针组和手法组造模成功后选取患侧头穴进行治疗,分别于造模前、造模后、治疗后即刻、第3天和第7天应用磁共振灌注加权成像观察各组家兔血肿周围脑组织rCBF、rCBV和MTT的变化。结果:与正常组相比,模型组MTT延长,rCBV和rCBF减小,血肿周围血流量下降(P<0.05)。留针组和手法组血肿周围脑血流灌注情况较模型组均有不同程度的好转,MTT缩短,rCBV和rCBF明显增加(P<0.05),手法组优于留针组(P<0.05)。结论:针刺可以改善急性脑出血血肿周围脑组织局部脑血流量的减少,平补平泻手法的应用可以进一步提高针刺疗效。

Objective： To observe effect of acupuncture with mild reinforcing and reducing manipulation by twirling and rotating on cerebral blood l ow of acute intracerebral hemorrhage rabbits. Methods： A total of 40 New Zealand rabbits were randomly divided into normal group, model group, retaining needle group and manipulation group, with 10 cases in each group. Self-arterial injection method had been used to copy the ICH rabbit model. After the model was successfully established, retaining needle group and manipulation group were treated on scalp acupoint of hemorrhage side. Quantitative analysis of PWI was used to observe the MTT, rCBV and rCBF of perihematoma at pre-modeling, after modeling, instant, 3 and 7days after acupuncture treatment. Results： After the ICH model was established, MTT extended, and rCBV and rCBF reduced signii cantly compared to normal group（P〈0.05）. After acupuncture therapy, MTT of retaining needle group and manipulation group shortened, and rCBV and rCBF increased（P〈0.05）. Manipulation group is more signii cant than retaining needle group（P〈0.05）. Conclusion： Acupuncture has therapeutic effect on ICH by improving regional cerebral blood l ow, and application of acupuncture manipulation could improve acupuncture effect.